Liquid Chromatograph Mass Spectrometer
Solving Complex Problems
High-sensitivity quantitation of intact catecholamines (CAs) in human plasma.
In clinical research, plasma catecholamines and their O-methylated metabolites (metanephrines) are measured as biomarkers for diseases such as hypertension, pheochromocytoma or neuroblastoma.
It is a challenging assay as the low physiological levels of CAs, physicochemical properties, and potential interferences require high sensitivity and specificity.
We developed a SPE-LC/MS/MS assay by using LCMS-8060 to detect catecholamines at ultra-high sensitivity without matrix interferences. As the measurement cycle time was 12 mins including the column re-equilibration the LCMS-8060 assay opens new possibilities for multiplexed sample analysis and higher sample throughput.
Detection of Norepinephrine, Epinephrine and Dopamine and their deuterated internal standards in plasma