Liquid Chromatograph Mass Spectrometer
Lipid Mediator Profiling
Lipid mediators represent a class of bioactive lipids that are produced locally through specific biosynthetic pathways in response to extracellular stimuli. Lipid mediators are involved in many physiological processes, and their dysregulations have been often linked to various diseases such as inflammation, infertility, atherosclerosis, ischemia, metabolic syndrome, and cancer. To help further our understanding of lipid mediators in the disease process we have created a method package designed to detect lipid mediators derived from arachidonic acid cascade.
Using the lipid mediator method package the LCMS-8060 detected arachidonic acids metabolites in human serum over a wide dynamic range from sub nM to μM concentrations. 5-HETE has shown the highest concentration which was 1 μM and the lowest was 12-HHT (0.5 nM). In case of 8-iso-PGF2α, its concentration was 0.1 nM.