Nexera Mikros

Microflow Liquid Chromatography Mass Spectrometry System

Robust and Easy-to-Use Microflow ESI Source

Micro-ESI 8060 + CTO-Mikros

Shimadzu’s Micro-ESI LCMS source has been designed for optimal sensitivity and ease of use. An X-Y adjustable stage and viewing camera allow for easy ESI spray needle adjustment. The column oven is mounted on the LCMS to minimize the volume between the exit of the column and the ESI needle. The Micro-ESI probe also features an ESI spray angle optimized for micro flow rates, and is compatible with LCMS-8045/8050/8060 systems. Mounted to the Micro-ESI source is the CTO-Mikros column oven. Stable column temperature, essential to retention time reproducibility, is ensured and columns up to 150 mm in length can be accommodated. To preserve the optimal source adjustment, the CTO-Mikros features an innovative snap-in column locking system, the UF-Link.

UF-Link™: Easy, Dependable Column Installation

When using microflow rates, even the smallest of dead volumes can cause significant diffusion and lead to loss of peak shape and sensitivity. Shimadzu’s UF-Link allows any user to correctly install a microflow column without disrupting the ESI spray needle position. A sure-sealing adapter allows the column to lock into the oven and make a zero-dead volume connection with the ESI needle. Any column up to 150 mm in length with standard fittings can be accommodated.


Connection Procedure

Attach the adapter to the column
Attach the adapter to the column.
Standard threads on the adapter make
it compatible with a wide variety of

Place the column in the UF-Link mechanism inside the oven.
Place the column in the UF-Link
mechanism inside the oven.

Complete the connection by swinging over the lever.
Complete the connection by swinging
over the lever.



Shimadzu LC pumps are the industry standard for long life and high performance. The LC-Mikros solvent delivery pump has completely new direct-drive engineering to deliver micro to semi-micro flow rates ranging from 1 to 500 μL/min. UHPLC system pressure tolerance of 80 MPa (11,600 psi) allows the use of every column type. The LC-Mikros pump is designed for microflow gradient elution, even at low percentages. In the example below, excellent gradient stability is shown using mobile phase compositions ranging from 1% to 90%. The LC-Mikros delivers stable retention times essential to the chromatographer.

Retention Time
Gly-Tyr 5.31 0.009 0.16%
Val-Tyr-Val 7.97 0.006 0.07%
Angiotensin II 9.32 0.015 0.16%
Leu enkephalin 9.71 0.011 0.11%
Met enkephalin 10.5 0.012 0.11%

Flowrate: 5 µL/min
Column:Shim-pack MC C18(0.3 mmID×150 mm)
*Obtained under our specified conditions.

Time (min) 1 11
13.1 25
Concentration B 1% 40% 90% 90% 1% 1%



The SIL-30AC autosampler delivers you excellent accuracy and reproducibility as well as extremely low carry-over. Its injection volume ranges from 0.1 to 50 μL and your precious sample has never wasted due to the total injection method SIL-30AC adapted.
It contributes to produce highly accurate results even if you had to use low injection volume (less than 1 μL). In case of micro flow rate with narrow column, optimum injection volume also gets lowered. It is also difficult to increase the injection volume, especially, if you would use a sample solvent with high elution power. In this case, sample must be diluted to weaken the sample solvents. Highly accurate micro volume injection capability of SIL-30AC allows you to use samples with high organic solvent contents without dilution.
System volume and gradient delay are minimized with the newly developed “programmable loop cut-off mechanism,” which automatically diverts the sample loop from the flow line using a high-pressure valve as soon as the sample in needle is drained. This is extremely important in minimizing microflow analysis run times.

For Research Use Only. Not for use in diagnostic procedures.

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