Cell Culture Media Analysis Platform
The Optimal Workflow Can Be Selected
The C2MAP system supports two modes: batch mode (ordinarily used), which gives priority to the pretreatment of the culture supernatant samples placed, and sequential mode in which pretreatment and analysis are performed alternately for each sample.
Performing only the sample pretreatment process is possible in batch mode (normal mode).
Supports a Wide Range of Measurement Compounds and Culture Supernatant Samples
The analysis method stored in the control software allows the simultaneous analysis of the following 95 components plus 2-Isopropylmalic acid.
It is confirmed that the C2MAP system can accommodate the follow ing culture media and culture media additives.
|Cell Type||CHO cells||iPS/ES cells||T cells||Mesenchymal Cell Type|
|Culture Media||BalanCD® CHO||AK03N||X-VIVO™ 10||MSCBM™|
|1xCD CHO||Essential-8™||X-VIVO™ 15||MesenPRO™|
|Additives||Fetal Bovine Serum (100% v/v)|
For results obtained via LC/MS/MS, temporal changes in each component can be graphed by the dedicated viewer software. Analysts can monitor variations in metabolites secreted from cells and culture media components during cultivation, as well as display graphs of component comparisons with samples from different culture series. As a result, the consumption and depletion of culture media components, and changes in the amounts of metabolic components secreted from cells, can be observed, thereby providing useful insights about the optimal culture conditions and assessments of cellular status.
Temporal Changes in Measured Components
Measured-component Comparisons among Different Culture Series
System Flexibility Creates New Value
The automatic sample pretreatment unit and the LC/MS/MS unit can be installed in different laboratories for separate use. For example, everything up to sample pretreatment can be performed in the culture laboratory, while a shared LC/MS/MS can be installed in a separate laboratory.
Providing Recommended Pretreatment Conditions for Multiple Medium Types
By optimizing the pretreatment conditions, it is possible to pretreat a variety of cell types in a single pretreatment condition.