Automated Pick and Collection Tool of Cell Colonies
Cloning of Genome Edited Cells via the Cell Colony Separation Method
After genome editing, the cell colonies are picked up and a clone of the target cells is obtained. Presented here is an example of picking HCT 116 cell line, a representative cell type used for genome editing. HCT 116 cells seeded in a 6 wellplate were picked with CELL PICKER and cultured for 6 days to confirm proliferation.
Cell Pickup During the Establishment of an iPS Cell Strain
CELL PICKER is used in the process of picking up the colony formed after the initialized genes are introduced.
In this case, picking up the iPS cell strain colony (strain 1231A3) maintained in culture is implemented with CELL PICKER. The cells were cultivated for 6 days after the cell pickup process. They were then immobilized, and immunostaining was performed. Expression of the undifferentiated markers Oct3/4 and Tra-1-60 was confirmed. In this way, the iPS cell colony picked up using CELL PICKER was maintained in an undifferentiated state and could evidently be cultured continuously.