Analytical Stability and Higher Detection
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Retention Time Reproducibility
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
Sample: Horse myoglobin 10pmol
Results show the subtraction chromatograms by SPD-M30A (PPSQ-51A/53A) and SPD-20A (PPSQ-31B/33B) connected tandemly after Edman degradation.