Gradient System


High-Sensitivity Analysis

Gradient separation enables detection of trace PTH-amino acids.

Analysis Stability

Equipped with a high-performance detector and a solvent delivery pump that provides excellent pumping performance even in the micro flowrate range.

High-Sensitivity Analysis

A high-sensitivity flow cell enables high-sensitivity detection of PTH-amino acids, which allows for sequential analysis using trace samples.

Analysis of Standard PTH-Amino Acid Mixture




Analysis Stability

This system has high analysis stability because it is equipped with a high-performance detector and a solvent delivery pump that provides excellent pumping performance, even in the micro flowrate range, combine to ensure high analysis stability. Because chromatograms with good reproducibility can be obtained, peaks detected in the previous cycle can be canceled by performing differential chromatogram processing. This enables easy identification of PTH-amino acids even from trace samples.

Analysis of Horse Myoglobin (10 pmol)


Example of High-Sensitivity Sequence Analysis Using Trace Samples

Erythropoietin Analysis

Erythropoietin is a hormone secreted by the kidney that stimulates red blood cell production.



Main Unit PPSQ-51A/53A
Reaction method Edman degradation
Reaction time PPSQ-51A: 46.5 min/cycle
PPSQ-53A: 48 min/cycle
Number of reactors PPSQ-51A: 1
PPSQ-53A: 3
Sample immobilization method Glass fiber disk (8 mm dia.) or PVDF membrane
Reactor temperature control range 10 °C above room temperature to 60 °C
Converter temperature control range 10 °C above room temperature to 70 °C
Number of reagents/solvents 7
Reagent/solvent delivery method Nitrogen gas pressure
Dimensions W510 x D500 x H540 mm
Weight PPSQ-51A: 43 kg
PPSQ-53A: 45 kg
Solvent Delivery Module LC-20AD
Flow rate setting range 0.0001 to 10 mL/min
Dimensions W260 x D420 x H140 mm
Weight 10 kg
Detector SPD-M30A
Wavelength setting range 190 to 700 nm
Dimensions W260 x D500 x H140 mm
Weight 12 kg
Oven CTO-20AC
Temperature control range Room temperature - 10 °C to 60 °C
Dimensions W260 x D420 x H415 mm
Weight 23 kg
Control PC
Windows 10 Pro 64 bit

Note: In the interests of product improvement, these specifications may change without notice.
Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.

Standard Configurations

PPSQ-51A System
Configuration PPSQ-51A (main unit)
LC-20AD (two)

PPSQ-53A System
Configuration PPSQ-53A (main unit)
LC-20AD (two)

Other Items Provided by User

Installation space Desktop: W1,800 x D600 x H600 mm min.
Weight: Approx. 120 kg
Power supply 120-230 VAC, 50/60 Hz, 1,500 VA max.
Nitrogen gas Purity: 99.9999% min.
A cylinder pressure regulator and gas tubing (10 m) are provided as standard accessories.
Exhaust equipment In order to provide an exhaust for gases produced by waste liquids, an exhaust tube must either be connected to exhaust equipment or vented outside. An exhaust tube (20 m) is provided as a standard accessory.

Note: The required installation space and power supply may change according to the PC and display used.

Installation Example

Installation Example


PPSQ-51A Single Reactor

PPSQ-51A Single Reactor

Offers highly cost-effective performance.

PPSQ-53A Triple Reactor (Supports consecutive analysis of 3 samples.)

PPSQ-53A Triple Reactor (Supports consecutive analysis of 3 samples.)

Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.

For Research Use Only. Not for use in diagnostic procedures.

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