Pesticide residues in fish tissue are caused by environmental contamination and present a potential hazard to human health. Many states and the EPA issue consumption advisories based on the observed levels of found pesticides, so it is important to monitor them accurately. Monitoring these pesticides levels in fish require analytical methods with good recoveries and low limits of quantification. Other sample preparation techniques such as accelerated solvent extraction (ASE) may cause complicated matrix interferences and use more solvent than SFE. In this study, we evaluated supercritical fluid extraction as a sample preparation technique for the extraction of pesticides from fish tissue, coupled with gas chromatography tandem mass spectrometry (GCMS/MS) to quantify 18 prevalent pesticides. For this poster, walleye fish with a low lipid content (%L=0.9) was studied.