A Method for Simultaneous Analysis of 174 Pesticides in Grape Using GC-MS/MS

With the development of science and technology, a great variety of pesticides have been used by more and more peasants and scientists. Simultaneous analysis of multi-residues such as hundreds of pesticides in agricultural products has always been a challenge. Since the detection limits for many pesticides has fallen to 10 μg/kg (10 ppb), more and more sophisticated analysis tools are required.
Due to its excellent sensitivity and selectivity, gas chromatography tandem mass spectrometry (GC-MS/MS) with multiple reaction monitoring (MRM) acquisition mode is utilized for analysis of residual pesticides in foods. The aim of this work was to develop and validate the fast method of simultaneous analysis of 174 pesticides in grape by GC-MS/MS in MRM acquisition mode. The samples were pretreated with the QuEChERS method, then subjected to GC-MS/MS. Each compound has 2 MRM transitions (primary for quantification, secondary for qualification), totally 348 transitions in 15 minutes. The established method is sensitive, repeatable and reliable for simultaneous analysis of 174 pesticides in grape samples.