Determination of methamphetamines in human saliva by GC-MS and two step injection on-column derivatization

To Analyze drugs using GCMS, samples are derivatized for high sensitivity, Typically samples are derivatized by placing them in a glass tube or vial and reacting them with a derivatizing agent, but the entire process requires about 30 minutes. However, the two-step injection mode provides a much easier method for derivatization, where the sample and derivatizing agent are each injected into the GC sample injection port and derivatized within column.