Simultaneous Determinations of 17 abused drugs and organophosphorus pesticides in human blood by GPC-GCMS

On-line gel permeation chromatography-gas chromatography/mass spectrometry (GPC-GCMS) is a unique technical to cleanup sample that reduce the time of sample preparation. GPC can ef ciently separates fats, protein and pigments from samples, due to this advantage, GPC-GCMS was widely used to pesticides analysis. In this work, a new method was developed for rapid determinations of 17 abused drugs and organophosphorus pesticides in the human blood by GPC-GCMS. The modi ed QuEChERS method was used for sample preparation. The human blood samples were extracted with acetonitrile, then was puri ed by PSA, C18 and MgSO4 to remove most of the fats, protein and pigments in samples, then after on-line GPC-GC/MS analysis which further removed macromolecular interference material, such as protein and cholesterol, the background interference brought about by the complex matrix in samples was effectively reduced. For all of analytes, recoveries in the acceptable range of 70 to 115% and repeatability (relative standard deviations, RSD)?5% (n=3) were achieved for both matrices at spiking levels of 0.05 and 0.5 μg/mL. The limitis of detection were 0.001 to 0.012 mg/L. The method is simple, rapid and characterized with acceptable sensitivity and accuracy to meet the requirements for the analysis of abused drugs and organophosphorus pesticides in the human blood.

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Content Type:
Paper
Document Number:
PO-CON1416E
Product Type:
Gas Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
Pittcon2014, Clinical research, Forensics, Healthcare, Toxicology, Drug test, GPC-GCMS
Language:
English
File Name:
slo214013.pdf
File Size:
850kb

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