Simultaneous Screening and Quantitation of Amphetamines in Urine by On-line SPE-LC/MS Method

Amphetamines belong to stimulant drugs and are also controlled as illicit drugs worldwide. The conventional analytical procedure of amphetamines in human urine includes initial immunological screening followed by GCMS confirmation and quantitation. With new SAMHSA guidelines effective in Oct 2010, screening, confirmation and quantitation of illicit drugs including amphetamines were allowed to employ LC/MS and LC/MS/MS, which usually does not require a derivatization step as used in the GCMS method. The objective of this study was to develop an on-line SPE-LC/MS method for analysis of five amphetamines in urine without sample pre-treatment except dilution with water. The compounds studied include amphetamine (AMPH), methamphetamine (MAMP) and three newly added MDMA, MDA and MDEA by the new SAMHSA guideline. Four potential interferences and PMPA (R) as a control reference were also included to enhance the method reliability in identification of the five targeted amphetamines from those structurally similar analogues which potentially present in forensic samples.

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Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Amphetamines, human urine, Clinical research, Forensics, Healthcare, Pharmaceutical, Life Science, Forensics, Toxicology, Drug test, LCMS-2020
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