Highly sensitive simultaneous quantitative analysis of estrone and equilin from plasma using LC/MS/MS
Equilin is an estrogenic steroid produced by horses. It has a total of four double bonds in the A- and B-ring. High concentration of equilin is found in the urine of pregnant mares. Equilin is one of the estrogens present in the mixture of estrogens isolated from horse urine and marketed as Premarin. Premarin became the most commonly used form of estrogen for hormone replacement therapy in the United States of America. Estrone is the major estrogen in Premarin (about 50%) and equilin is present as about 25% of the total. Estrone is a major estrogen that is normally found in women. Equilin is not normally present in women, so there has been interest in the effects of equilin on the human body.
Here, an LC/MS/MS method has been developed for highly sensitive simultaneous quantitation of estrone and equilin (structure shown in Figure 1) from charcoal stripped plasma using LCMS-8060, a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan.
*Not available in the U.S.
- Content Type:
- Paper
- Document Number:
- PO-CON1746E
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- estrone, equilin, Clinical research, Forensics, Healthcare, LCMS-8060
- Language:
- English
- File Name:
- apo117077.pdf
- File Size:
- 625kb