Quantitation of plasma metanephrine and normetanephrine by derivatization using an integrated LC-MS/MS analyzer equipped with fully-automated sample preparation device
- Although LC-MS/MS analysis manifests high repeatability in measurement, overall reproducibility of an assay is compromised by errors associated with manual sample pretreatment. This also hinders standardization of assay across multiple laboratories.
- Derivatization of is employed in LC-MS/MS analysis to achieve better chromatographic separation or to enhance favorable detection in MS. However, it involves series of reagent addition and vortexing, making the pretreatment prone to errors and hence low reproducibility.
- In this investigation, we evaluated tested whether or not automated sample preparation device can be employed to carry out complex sample pretreatment procedures, such as required for chemical derivatization. For model experiment plasma metanephrine and normetanephrine was derivatized by reductive amination as shown in Fig. 1 to improve reversed phase column retention.
*Not available in the U.S.
- Content Type:
- Paper
- Document Number:
- PO-CON1751E
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- metanephrine, normetanephrine, Clinical research, Forensics, Healthcare, Clinical research, LCMS-8060
- Language:
- English
- File Name:
- apo21768.pdf
- File Size:
- 411kb