Maximizing Efficiency for UHPLC LC-MS/MS Method Development in clinical drug monitoring

Traditional method development in HPLC is extremely time consuming. The combination of automated HPLC and MS method development allows the creation of complete LCMSMS methods within a single day. In this study we show an automated method scouting procedure including the search for optimum column and mobile phase and the improvement of various parameters like flow rate, column temperature and gradient conditions. The final HPLC method validation is also software assisted. The combination with the fully automated MRM-optimization by flow injection allows a fast development of a final method for the analysis of clinical drugs. Here we show the successful establishment of an automated generated method for the separation, identification and quantification of a mixture of drugs.

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Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Method Scouting, UHPLC, clinical drug monitoring, Clinical research, Forensics, Healthcare, Pharmaceutical, Life Science, Drug discovery, Clinical research, LCMS-8040
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