Analysis of 25-OH Vitamin D2 / D3 in Plasma and Serum by LCMS-8040 using ClinRep^® LC-MS/MS Complete Kit MS7000

Vitamin D measurement has become an important component in clinical assays largely because deficiency is associated with a number of disorders such as rickets, osteomalacia and osteoporosis. When serum concentration falls below 20 ng/mL osteoporosis can result, with normal levels ranging from 20-50 ng/mL. Developments in high pressure fast chromatography LC-MS/MS have now enabled on-line sample preparation methods and analysis times in less than 3 minutes. In these experiments tandem mass spectrometry was used to quantify 25-OH Vitamin D2 / D3 using a kit containing standard compounds and pre-optimised method parameters.