Measurement of immunosuppressants, Tacrolimus, Sirolimus, Everollmus and Cyclosporine A from whole blood using on-line SPE and LCMS-8030

Immunosuppressants are an important class of compounds which are commonly used by transplant recipients to avoid organ rejection. In addition, they are used for the treatment of immune-mediated diseases or disorders of the immune system and non-autoimmune inflammatory reactions such as heavy allergic asthma. The therapeutic concentration range of these compounds, typically narrow, requires careful monitoring from whole blood to ensure the correct patient dosage. In these experiments on-line separation of whole blood was performed, analyzing the immunosuppressant compounds tacrolimus, sirolimus, everolimus and cyclosporine A (CSA) by liquid chromatography tandem mass spectrometry LC-MS/MS.