Serotype-Level Bacterial Discrimination Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
We successfully achieved serotype-level discrimination of enterohemorrhagic Escherichia coli using a proteotyping method (the S10-GERMS method) with markers consisting of ribosomal proteins detected during bacterial measurements using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). In the future, this technique could be utilized as a routine method for the discrimination of bacteria at levels finer than the species level, which has thus far not been possible with conventional MALDI-TOF MS fingerprinting-based microbial identification methods.
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- Content Type:
- Application
- Document Number:
- C146-E331
- Product Type:
- Mass Spectrometry, Life Science Lab Instruments, MALDI-TOF Mass Spectrometry
- Keywords:
- food-poisoning pathogens, MALDI-TOF MS, proteotyping, S10-GERMS, Strain Solution Ver. 2, Clinical research, Forensics, Healthcare, Food and Beverages, Pharmaceutical, Life Science, AXIMA
- Language:
- English
- File Name:
- jpo316098.pdf
- File Size:
- 493kb