Sensitive HPLC Method for Quantitative Analysis of Mandelic Acid, Hippuric Acid, Methyl Hippuric Acid in Human Urine

Urine metabolites such as Hippuric Acid (HA), o-Methyl Hippuric Acid (M-HA) and Mandelic Acid (MA) are indicators of exposure to solvents Toluene, Xylene and Styrene respectively. Frequent exposure to these solvents may cause damage to the central nervous system. To determine the level of exposure, urine samples can be analysed by determining the HA, M-HA and MA concentration per creatinine mass of an individual. Normal level of HA for an unexposed subject is 1g/g Creatinine while M-HA should not be present. The threshold limits for the exposed subject are 1.6g HA/g Creatinine, 1.5g M-HA/g Creatinine2 and 0.8g MA/g Creatinine. HPLC can simultaneously analyse HA, M-HA, MA and creatinine in urine using simple sample preparation.

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Liquid Chromatography
HPLC, Urine, Metabolites, Clinical research, Forensics, Prominence HPLC
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