HPLC method scouting system using ultra high performance liquid chromatography coupled to single quadrupolemass spectrometer

Traditionally, the process of LC/MS method development has been a time consuming as well as labor intensive task. Optimization of separation conditions that also maximize mass spectrometry sensitivity requires a methodical approach that can involve multiple experiments involving column switching and mobile phase screening. In this study a UHPLC based method scouting system was used to dramatically shorten total experiment time compared to conventional approaches. This enabled multiple separations based on the combination of different mobile phase and multiple columns allowing comprehensive investigation of analytical conditions. Mass spectrometry sensitivity, also influenced by mobile phase pH buffer, can be optimized via this approach. Method scouting was applied to the simultaneous analysis of geno-toxic impurities, several amines and acidic compounds using UHPLC coupled to a single quadrupole LC/MS instrument.