A novel approach to the analysis of multivitamin by online supercritical fluid extraction/supercritical fulid chromatography

Vitamin is a series of basic trace substances which could maintain normal life forms of the animal body. Due to the chemical structure, fat-soluble vitamins such as vitamin A, vitamin E, etc. have strong hydrophobicity, low solubility in polar organic solvents. The analytical methods of those compounds are various, such as vitamin A by reversed phase liquid chromatography (RP-HPLC), vitamin D by normal phase liquid chromatography (NP-HPLC), and vitamin E normally used gas chromatography. Because of original method diversity, it is difficult to develop a new method of simultaneous analysis for fat-soluble vitamins. Supercritical Fluid Chromatography (SFC) is an unconventional chromatographic separation technology by using supercritical fluid and a small amount of modifier as mobile phase. Supercritical CO₂ (scCO₂) with its character of safe, inexpensive, non-toxic, facile, chemical inertness and other factors become the main mobile phase of SFC. Supercritical fluid (scCO₂) with low viscosity, high diffusivity and solubility characteristics is used in a wide variety of fields. Nexera UC Online SFE - SFC system is the latest products of supercritical fluid chromatograph in Shimadzu, which realized SFE (supercritical flow extraction) and SFC online combination, and simplify and unify the pretreatment method with high automation, extraction efficiency, and repeatability.In this study, a simultaneous analytical method for fat-solvable vitamins in drug and health care food was developed by using Nexera UC. It provides effective analysis and detection means for a variety of fat-soluble vitamins, and can be the reference for the quantitative study of this kind of material.