UHPLC method transferred from the USP monograph method was demonstrated for amoxicillin oral suspension as a representative example. In the USP monograph method, normally an analytical condition for HPLC is designed using traditional HPLC separation which has a relatively longer analytical cycle. UHPLC has good advantage as less analytical cycle in several minutes than traditional HPLC. For amoxicillin analysis, around 20 minutes analytical cycle by traditional HPLC could be transferred to approximately 4 minutes analytical cycle by UHPLC in almost one-fifth less time with almost same chromatographic separation. Amoxicillin as an active pharmaceutical ingredient could be clearly identified using UHPLC.