Ultrahigh Separation Analysis of Nucleotides

Nucleotides, building blocks of DNA and RNA, are compounds where phosphate(s) bond to a nucleoside that is composed of a nucleobase and sugar. In general, adenine (A), cytosine (C), guanine (G), thymine (T), and uracil (U) are examples of nucleobases, and ribose and deoxyribose are examples of sugar.This document introduces an example of ultrahigh separation analysis performed on nucleotides using Nexera, which is resistant to 130 MPa, and a Shim‐pack XR‐ODS III column (particle size 2.2 μm), resistant to 100 MPa.