Ultrafast Analysis of Water-Soluble Vitamins

Water‐soluble vitamins such as thiamine (vitamin B1) and riboflavin (vitamin B2) are generally analyzed by reverse‐phase ion‐pair chromatography. This document introduces an example of ultrafast analysis performed on water‐soluble vitamins by gradient elution using Nexera and a Phenomenex Kinetex C18 column (particle size 2.6 μm, a core‐shell column where a 0.35 μm porous membrane is combined with a 1.9 μm solid core).