Analysis of Steviol Glycosides in Stevia Extract

Steviol glycosides are natural sweeteners, and are used as food additive. The ADI was established for steviol glycosides at the 69th JECFA conference held in June 2008, and starting with the United States and the European Union, permission for its use has actively spread to other countries. Here, we introduce an example of analysis of the 9 major steviol glycosides in the stevia extract by reversed phase chromatography.