Improved R&D Efficiency Through Speedier Method Development (3)

Method scouting is the search for analytical conditions (column, mobile phase, and gradient settings) that optimize an LC separation. When used with a UHPLC (ultra high performance LC) system, the time to produce a robust, validated method is greatly shortened, which leads to an increased interest in and application of UHPLC systems for method development. In this report, we introduce an example of such scouting using the Nexera Method Scouting System together with its specialized Method Scouting Solution software to conduct a search for simultaneous analytical conditions to be used in analysis of 13 cephem antibiotic substances.