Orotic acid, a heteroaromatic compound discovered in whey, is a major precursor of the nucleic acid pyrimidine, and has been attracting attention due to its diverse physiological effects. On January 23, 2012, the Pharmaceutical and Food Safety Bureau of the Japanese Ministry of Health, Labour and Welfare published a notification (No. 0123-3), and orotic acid, orotic acid potassium salt, and magnesium salt were added to the list of items specified as "ingredients that are not considered as drugs unless drug efficacy is advocated". As a result, its increased use in health foods, nutritional supplements, etc. can be expected. Here, we introduce an example of analysis of orotic acid in yogurt by reversed-phase chromatography.