High Sensitivity Profiling of Glycans in Antibody Drugs Using RF-20Axs
Glycans in antibody drugs may play roles in the antigenicity, pharmacokinetics and high-order structural stability of drugs, which could adversely affect their safety and effectiveness. It is therefore necessary to investigate which glycans are present in antibody pharmaceuticals. Non-uniformity of glycans due to the instability of culture conditions used for antibody drugs is a concern which requires rigorous management of the production process. Here, using the core-shell column AerisTM PEPTIDE XB-C18 and the Nexera X2 ultra high performance liquid chromatograph, we introduce an example of the analysis of antibody drug glycans. For detection, the world-leading sensitivity fluorescence detector, the RF-20Axs was used.
- Content Type:
- Application
- Document Number:
- LAAN-A-LC-E230A
- Product Type:
- Liquid Chromatography
- Keywords:
- Glycans, Antibody drugs, PA-derivatization, Pyridylamination, RF-20Axs, Fluorescence detector, Aeris PEPTIDE, Core-shell column, Pharmaceutical, Life Science, DMPK, ADME, Safety testing, Nexera X2
- Language:
- English
- File Name:
- jpl214001.pdf
- File Size:
- 1,008kb