Comprehensive Two-dimensional Liquid Chromatography for Determination of Polyphenols in Red Wines

A comprehensive two-dimensional liquid chromatography method was developed and applied to the determination of polyphenols in red wines. To fulfil such a task, a micro cyano and a partially porous octadecylsilane columns were employed in the first and the second dimension, respectively in combination with photodiode array and mass spectrometry (LCxLC-PDA-MS) detection. To increase the peak capacity values by using RP modes in both dimensions, a comparison of a conventional full-in-fraction and shifted second dimension gradient was carried out. The separation capabilities of the comprehensive LC approaches tested allowed the analysis of such a complex natural sample without any pre-treatment to effectively reduce the interferences coming from the matrix.