Continuous vs. Segmented Second Dimension System Gradients for Comprehensive Two-dimensional liquid Chromatography of Sugarcane (Saccharum spp.)

Polyphenols in sugarcane (Saccharum spp.) leaf extracts were analyzed by comprehensive two-dimensional liquid chromatography in combination with photodiode array and mass spectrometry (LCxLC-PDA-MS) detection; a micro cyano and a partially porous octadecylsilane column were employed in the first and the second dimension, respectively. Even using RP mode in both dimensions, a satisfactory degree of orthogonality was achieved by employment of different approaches of gradient elution mode in the second dimension. By means of the investigated set-up, a total of 38 polyphenolic compounds were detected with the complementary data of PDA, MS and an in-house database.