Rapid Analysis of 2,4-DNPH-Derivatized Aldehydes and Ketones Using the Prominence-i with a Shimpack XR-ODS Column

Application News No. L268 presented a rapid analysis of 2,4-DNPH (2,4-dinitrophenylhydrazine)-derivatized aldehydes and ketones using the LC-2010 integrated high-performance liquid chromatograph with a Shim-pack FC-ODS column. As the goal of this study was to obtain a shorter the analysis time than that using the previous method, we investigated the run conditions for 2,4-DNPH-derivatized aldehydes and ketones using the new Prominence-i integrated high-performance liquid chromatograph with a 2.2 μm particle size Shim-pack XR-ODS column.
Here we present the results of simultaneous analysis of thirteen 2,4-DNPH-derivatized aldehyde and ketone standards run on a conventional 5 μm particle column, and an example of rapid analysis with a 2.2 μm particle column.