Analysis of Organic Acids in Culture Medium Using Post-Column pH Buffering Organic Acid Analysis System

The shift from emphasis on fossil fuels in recent years has been emphasized with increasingly active research into the bioproduction and manufacture of new energy sources and chemical products. In the field of bioproduction, microbial cells serve as factories. In the process by which sugars are metabolized to produce the desired compounds in the culture medium, organic acids such as pyruvic acid, lactic acid, acetic acid, formic acid and succinic acid are also produced as metabolites. Accurate identification and quantification of these organic acids makes it possible to accurately grasp the metabolic state of the cell, for example elucidation of the flow of carbon introduced into the system.
Here, we introduce an example of analysis of organic acids in a culture medium using post-column pH buffered electrical conductivity detection in combination with a dual column oven, a system that provides both improved separation and high-selectivity detection.

Content Type:
Application
Document Number:
LAAN-A-LC-E267
Product Type:
Liquid Chromatography
Keywords:
Organic Acids, Culture medium, Bioproduction, Pyruvic acid, Lactic acid, Biomass, New energy, Microbial, Metabolomics, Dual Column Oven, Shim-pack SCR-102H, Post-Column pH Buffering, Conductivity detector, Pharmaceutical, Life Science, DMPK, ADME, Safety testing, Prominence, CTO-20AC
Language:
English
File Name:
jpl215022.pdf
File Size:
889kb

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