Comprehensive 2D Separation of Triglycerides in Vegetable Oil with ELSD/LCMS-IT-TOF Detection

When conducting comprehensive two-dimensional liquid chromatography, different separation modes are generally selected for the first and second-dimension separations, and depending on the differences in separation selectivity between these dimensions, improved separation is typically seen for components that are difficult to separate in a single, one-dimensional analysis.
Here, using borage oil as a sample that contains many triglycerides, micro-scale separation was conducted in the first separation using a silver column (normal phase conditions), and reversed phase separation was conducted in the second dimension by using a two-liquid gradient with non-aqueous organic solvents. Detection was conducted using a combination of an evaporative light scattering detector (ELSD) and an ion trap time-of-flight mass spectrometer (LCMS-IT-TOF).