Comprehensive 2D Separation of Lipid Species in Mussels Using the Nexera-e System Combined with LCMS-IT-TOF Detection

In a typical analysis of lipids, the whole lipids extracted from a biological sample are fractionated based on lipid classes using normal-phase chromatography or hydrophilic interaction liquid chromatography (HILIC), and then LC/MS analysis is performed for each fraction obtained. One drawback of this method is that it requires a lot of time. Comprehensive two-dimensional (2D) liquid chromatography combines two independent separation modes. The different separation selectivities of the two dimensions are combined to improve the separation of components that are difficult to separate by single dimensional analysis. This article presents an example separation of lipids in mussels using the Nexera-e, which is an effective system for the comprehensive separation of a large number of lipid molecules.