Effective Analysis Management Achieved by Method Transfer between HPLC and UHPLC

High performance liquid chromatography (HPLC) is widely used for the qualitative and quantitative analysis. In recent years, ultra-high performance liquid chromatography (UHPLC) has been developed significantly. Facilities that have both UHPLC and HPLC systems often apply newly established UHPLC method to ordinary HPLC as well as applying existing HPLC method to UHPLC. During such inter-system method application, the method must be modified properly to meet the requirements from another system. This modification is called "method transfer". Method transfer requires to modify existing parameters such as flow rate and/or time program.
Here, we describe the relationship between column dimensions including particle size of packing material and analytical conditions, then method transfer from HPLC to UHPLC for high speed analysis and that from UHPLC to HPLC for generalization of analytical conditions as well as that from Shimadzu's system to other vendor’s. We also describe the Shimadzu integrated LC system "Nexera-i MT" that supports method transfer and ACTO (Analytical Conditions Transfer and Optimization) Function equipped in the latest LabSolutions LC software.