Improving Method Transfer by Adjustment of Gradient Delay
In facilities that have number of high performance liquid chromatography (HPLC) systems, an existing method that gives proper result by one HPLC system is often applied to other HPLC systems (method transfer). However, due to the difference in system volume, pump characteristics, and liquid delivery mechanisms among systems, method transfer can yield different results even though the same method is used.
In this report, we first explain the gradient delay due to the system volume difference and the effect of the delay on separation. We then show an example of method transfer with adjustment of gradient delay using alkylphenones and UV absorbents as samples. We also describe the integrated liquid chromatograph (LC) system "i-Series" that supports to optimize method transfer and the newly designed function of "Analytical Conditions Transfer and Optimization (ACTO)" equipped in the latest version of LabSolutions software.
- Content Type:
- Application
- Document Number:
- C190-E204
- Product Type:
- Liquid Chromatography
- Keywords:
- HPLC, method transfer, ACTO, Pharmaceutical, Life Science, i-Series
- Language:
- English
- File Name:
- jpl216022.pdf
- File Size:
- 163kb