Increased Analysis Throughput by Overlapped Injection Using the SIL-40 series Autosampler

The use of high-speed analysis methods is now widespread because of improvements in separation efficiency due to the use of ultra-small particles in column packing materials and surface porous particles, and the associated higher pressure resistance and lower size of the instruments. However, to improve the overall analysis throughput, it is necessary to effectively utilize the sample injection time and the times when peaks are not eluted, periods outside the time for the chromatogram to become visible. The minimum injection time of the SIL-40 series autosampler (SIL-40) is 7 seconds or less, which is extremely effective for improving the throughput of the overall analysis. In addition, by performing a simple setting and injecting the sample for the next analysis during the current analysis (overlapped injection), the whole analysis cycle time be can be shortened and throughput increased.
Here, we describe how to increase efficiency in size exclusion mode, where improving throughput is normally difficult, by utilizing overlapped injection.