Improving Peak Shape Using the Automatic Pretreatment Function (Co-Injection) in the SIL-40 Series Autosamplersceted Data Using the Dual Injection Function in the SIL-40 Series Autosampler

If a sample solvent selected in accordance with the solubility of a compound has a higher elution strength than the mobile phase, this can have a negative impact on the chromatogram. For example, if the ratio of organic solvent in a sample solvent is heightened in order to dissolve a compound with low polarity, this can have a detrimental effect on peak shape for quickly eluted compounds in reverse-phase chromatography, and even compromise the reliability of quantitative results. This article illustrates the use of the co-injection function, one aspect of the automatic pretreatment functionality included as standard in autosamplers in the Nexera™ series (SIL-40 series), to improve peak shape by injecting a solvent with a weak elution strength simultaneously with the sample in order to reduce the effect of the sample solvent. The co-injection function can be specified easily via the LabSolutions workstation software.