Analysis of Polysorbate 80 in IgG Aqueous Solution by Online SPE Using Shim-pack MAYI Column - Part 1

As the Shim-pack MAYI series, with its packing pore outer surface coated with a hydrophilic polymer, offers a line-up of online SPE columns that can quickly eliminate proteins, when combined with a column switching HPLC, a variety of components can be analyzed in a seamless flow from deproteinization to analysis. Here, we introduce an example of analysis of the polysorbate 80 surfactant, widely used as an additive to prevent protein aggregation and adsorption, and to increase protein solubility in a protein formulation.