Analysis of Polysorbate 80 in IgG Aqueous Solution by Online SPE Using a Shim-pack MAYI Column - Part 2

Polysorbates play a role in maintaining the stability of proteins to prevent their denaturation, so they are often added to protein formulations. Therefore, evaluation of the quality and stability of these formulations also requires evaluation of the quality of the polysorbate.
In Application News No. L486, a method in which automated deproteinization can be conducted followed by quantitative analysis of polysorbate 80 in an antibody model sample was presented.
Here, using this system for higher resolution analysis, we conducted detection and mass spectral measurement of possible by-product components of polysorbate 80.