LC/MS/MS Method for Quantitative Determination of Ethinyl Estradiol in Human Plasma
An ultra sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for quantitative analysis of oral contraceptive Ethinyl estradiol (EE) has been developed in human plasma using UHPLC NEXERA coupled to LCMS-8040 triple quadrupole mass spectrometer and described here. A two stage sample clean-up and also off-line derivatization using Dansyl chloride enabled ultra sensitivity up to 1.0 pg/mL using 750 μL of human plasma. The method was found to be linear in the range of 1 -200 pg/mL.
- Content Type:
- Application
- Document Number:
- AD-0078
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- Ethinyl Estradiol, Human Plasma, Ultra Sensitive, Pharmaceutical, Life Science, LCMS-8040 UFMS
- Language:
- English
- File Name:
- apo115096.pdf
- File Size:
- 271kb
