Quantitative Analysis of Residual Artificial Sweeteners in Surface Water by Highly Sensitive LC/MS/MS Method

Artificial sweeteners, being low caloric sugar substitutes, are widely used in food and beverages for decades. Researches on environmental occurrence and eco-toxicological effects of artificial sweeteners have increased in recent years. Artificial sweeteners are found in surface waters and wastewaters at levels from ng/L to μg/L, which are classified as new emerging, environmental contaminants. The artificial sweeteners which have been studied most in this field are acesulfame (ACE), cyclamate (CYC), saccharin (SAC) and sucralose (SUC). However, pre-concentration of water sample is often required because of the needs for detection of trace levels of the compounds. A high sensitivity triple quadrupole system LCMS-8060 with heated ESI is coupled to a Nexera UHPLC system was employed in aiming at achieving ultra-high sensitivity for direct quantitation of five artificial sweeteners in surface waters and drinking waters. A biphenyl column obtained from Phenomenex was used, which is described with features of enhanced selectivity and aqueous stability. A gradient elution program of 6 minutes was developed. The LODs of the method for the target artificial sweeteners are better than 2.5 ng/L, except for sucralose (SUC). The analysis results were based on an injection volume of 10 μL. With an increased injection volume of 30 μL, the sensitivity of the method is expected to increase to be able to achieve a LOD of 10 ng/L for SUC.

Content Type:
Application
Document Number:
AD-0126
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
Water, Surface Water, Artificial Sweeteners, Sweeteners, Aspartame, Acesulfame, Cyclamate, Saccharin and Sucralose, Environment, Discharge Water, Groundwater, Environment Water, LCMS-8060
Language:
English
File Name:
apo117022.pdf
File Size:
413kb

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