Multi-residual quantitative analytical method for antibiotics in sea food by LC/MS/MS

Antibiotics are widely used in agriculture as growth enhancers, disease treatment and control in animal feeding operations. Concerns for increased antibiotic resistance of microorganisms have prompted research into the environmental occurrence of these compounds. Assessment of the environmental occurrence of antibiotics depends on development of sensitive and selective analytical methods based on new instrumental technologies.
LC/MS/MS method has been developed for quantitation of multi-residual antibiotics (Table 1) from sea food sample using LCMS-8040, a triple quadrupole mass spectrometer from Shimadzu Corporation, Japan. Simultaneous analysis of multi-residual antibiotics often exhibit peak shape distortion owing to their different chemical nature. To overcome this, autosampler pre-treatment feature was used.