Simultaneous analysis of supernatant components of cell culture using triple quadrupole LC/MS/MS

Fermentation for the production of biofuels, biopharmaceutics or other compounds requires routine monitoring of medium conditions such as pH, dissolved gas, carbon source and nitrogen source for optimization and control of the fermentation process. However, culture media also consist of various other biologically important compounds such as vitamins, nucleic acids and other primary metabolites, which would lead to more detailed understanding of the bioprocess if monitored altogether. To meet the demand for comprehensive analysis of medium components, we optimized analytical conditions and developed "Method Package for Cell Culture Profiling" for monitoring relative abundance of 95 compounds. Using this method package, we demonstrated the change in abundance of culture supernatant (bacteria and streptomyces cells) components for over the culture periods.