Screening of 25-OH Vitamin D2,D3 using Shimadzu LCMS-8040
Hypovitaminosis D is a relatively common problem traditionally manifesting in the elderly population and in people with severe liver or kidney disease. Inadequate levels of vitamin D can lead to increased risk of cancer,
diabetes mellitus, chronic pain, and hypertension. Vitamin D2 (ergocalciferol) and D3 (cholecalciferol) may come from exogenous sources, but only vitamin D3 is produced in the skin from 7-dehydrocholesterol upon exposure to sunlight. Vitamin D2 and D3 are metabolized to 25-hydroxyvitamin D [25(OH)D2 and 25(OH)D3] in the liver and subsequently converted to the biologically active 1,25-dihydroxyvitamin D form in the kidneys. Circulating levels of 25-hydroxyvitamin D have been demonstrated to be the most precise marker of a patient’s vitamin D status. Various methodologies including HPLC-UV, HPLC-EC, RIA, automated chemiluminescence immunoassays have been described for the measurement of 25-OH-VitD but just LCMSMS offers a simple, linear and reproducible result. We evaluated Shimadzu Triple Quadrupole LCMS-8040
instrument using different commercial kit avaible for detection of 25-OH VitaminD and a home-brew method using a considerable number of biological sample.
- Content Type:
- Paper
- Document Number:
- PO-CON1358E
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- HPLC2013, Vitamin D2, Vitamin D3, hydroxyvitamin, Pharmaceutical, Life Science, DMPK, ADME, Safety testing, LCMS-8040
- Language:
- English
- File Name:
- imo113084.pdf
- File Size:
- 359kb