A triacylglyceride (TAG) is an ester derived from glycerol and three Fatty Acids (FA). TAGs are found in both plant oils and animal fats. Different varieties of oils are used for culinary purposes across the globe. Hence, their characterization is important for nutritional reasons. However, separation and identification of components of the complex mixtures of TAG that constitute fats and oils is a challenging. Characterization of the TAGs present in edible oils requires acquisition of precursor ion m/z by full scan MS mode. Full scan MS spectra are generally complicated because of presence of varieties of TAGs. Furthermore, it is necessary to fragment all these precursor ions so as to understand fatty acid composition of TAGs. Selecting these precursor ions manually and creating a method to fragment them is also a tedious task.
This task is simplified by Synchronized Survey Scan (SSS) available in LCMS-8040 with LabSolutions software. Here, product ion spectra of all the major precursor ions are obtained as a dependent event of full scan MS in a single analysis, attributed to ultrafast scanning speed of 15000 u/sec of LCMS-8040.