Multiresidue pesticide analysis in crude food extracts using AOC-MEPS and LC/MS/MS

In the method titled "Multiresidue Method using QuEChERS followed by GC-QqQ/MS/MS and LC-QqQ/MS/MS for Fruits and Vegetables" published by the European Union Reference Laboratory (EURL), 138 pesticides were selected as target analytes. Of these 138 pesticides, the EURL analyzed and validated 66 pesticides by GC-QqQ and the remaining 72 pesticides by LC-QqQ. In this study, we selected 120 compounds which could be measured by LC/MS from the 138 compounds, and these compounds were analyzed in a single analysis using MRM. This was achievable due to the fast dwell (5 msec), pause times (1msec) and rapid polarity switching (15 msec) of the triple quadruple mass spectrometer.
Each MRM was optimized and the required LOQs for control of pesticide residues in food (10ppb, maximum residual limit in food matrices) were achieved for all compounds. By using the AOC-MEPS system, calibration curves with excellent linearity in the range of 5-1000ppb and good recovery were obtained for 60 % of target compounds. However, water-soluble compounds that are not retained much on the C18 column could not be trapped on the PVDB sorbent material. Therefore, we tested a different type of MEPS cartridge, which was packed with an ion exchange polymer as a sorbent bed for improving the recovery of water-soluble compounds. The real sample spiked with 10 ppb standards was prepared for the recovery test in the vegetable matrices. The majority of the target compounds which showed a good linearity in the calibration curve were successfully detected and presented the good recoveries by the AOC-MEPS from vegetable matrices, in the range 70-120%.

Content Type:
Paper
Document Number:
PO-CON1305E
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
pesticide residues in food, LC/MS/MS, Food and Beverages, Food safety (Residues, Contaminants), LCMS-8040
Language:
English
File Name:
jpo113040.pdf
File Size:
207kb

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