Rapid analysis of triglycerides and fatty acids in food oils using DART-MS with high-speed polarity switching

Conventionally, common ways to analyze triglycerides have been by using liquid chromatograph or gas chromatograph. Some of the problems with those methods have been the cumbersome sample prep, lengthy analysis time and memory effect. Previous reports on DART-MS analysis of triglycerides and fatty acids have involved separate acquisitions of positive spectra and negative spectra, but this time we have successfully applied the ultra-fast polarity switching to carry out high-throughput parallel analyses of fatty acid compositions in triglycerides and free fatty acids using Direct Analysis in Real Time (DART) mass spectrometry.