Accelerating quantitative proteomics analysis with Shimadzu UFMS and Skyline

The Key challenge for quantitative proteomics researchers is to accurately quantitate proteins and peptides in complex biological systems with high sensitivity. Multiple reaction monitoring (MRM) is the main current approach for highly confident protein and peptide quantification. In a method development of quantitative proteomics, thousands of MRM are needed to be monitored to exhaustively pursue an optimum condition.
Skyline1 can simplify the workflow for both method set up and data processing. On the other hand, in typical triple quadrupole mass spectrometers, it is mandatory for users to divide the experiment into multiple methods to monitor over a thousand MRMs with adequate sensitivity.