Measurement of Homocysteine in Plasma with LCMS-8040

Homocysteine is used as an indicator when analyzing for activity of enzymes involved in the methionine metabolism pathway, such as methionine synthase and methylenetetrahydrofolate reductase. Here we describe an example analysis for homocysteine performed using an LCMS-8040 high-performance liquid chromatograph triple quadrupole mass spectrometer and employing an analytical protocol used by the Mass Spectrometry, Clinical Chemistry and Pharmacology Lab. of Meyer Children's Hospital (Florence, Italy).